Part:BBa_K2683020
Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) in pSB1C3
Phenylalanine tRNA synthetase (PheRS) is responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. PheRS has both a alpha and beta subunit [1]. The part is codon optimized for use in Escherichia coli , contains a N-terminal hexahistidine tag with a serine glycine linker and is under the regulation of a T7 Promoter, medium strength RBS and double terminator. The part was originally designed by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge) and was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443014).
The part was improved by placing it into the pSB1C3 plasmid.
Figure 1- Overexpression of PheRS alpha protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced PheRSalpha protein can be seen at about 38kDa.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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